British pair Emma Raducanu and Harriet Dart lost in straight sets in the first round of the Madrid Open.
Raducanu, 21, was beaten 6-2 6-2 by Argentine qualifier Maria Lourdes Carle.
Dart, who won two qualifying matches to reach the main draw, held a 4-0 lead in her second set against Spain's Cristina Bucsa but lost 6-4 6-4.
In the men's draw, Britain's Jack Draper will begin his tournament against Australian qualifier Thanasi Kokkinakis later on Wednesday.
Raducanu was set to play Karolina Pliskova in her opening match but the former world number one pulled out of the tournament because of a wrist injury.